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Getting started in cryo-EM(으)로 돌아가기

Getting started in cryo-EM, 캘리포니아 공과대학

(220개의 평가)

About this Course

This class covers the fundamental principles underlying cryo-electron microscopy (cryo-EM) starting with the basic anatomy of electron microscopes, an introduction to Fourier transforms, and the principles of image formation. Building upon that foundation, the class then covers the sample preparation issues, data collection strategies, and basic image processing workflows for all 3 basic modalities of modern cryo-EM: tomography, single particle analysis, and 2-D crystallography. Philosophy: The course emphasizes concepts rather than mathematical details, taught through numerous drawings and example images. It is meant for anyone interested in the burgeoning fields of cryo-EM and 3-D EM, including cell biologists or molecular biologists without extensive training in mathematics or imaging physics and practicing electron microscopists who want to broaden their understanding of the field. The class is perfect as a primer for anyone who is about to be trained as a cryo-electron microscopist, or for anyone who needs an introduction to the field to be able to understand the literature or the talks and conversations they will hear at cryo-EM meetings. Pre-requisites: The recommended prerequisites are college-freshman-level math, physics, and biochemistry. Pace: There are 14.5 hours of lecture videos total separated into 40 individual “modules” lasting on average 20 minutes each. Each module has at the end a list of “concept check” questions you can use to test your knowledge of what was presented. As the modules are grouped into seven major subjects, one reasonable plan would be to go through one major subject each day. That would mean watching a couple hours of lecture and spending another hour or so thinking through the concept check questions each day for a week. Another reasonable plan would be to go through one module each day for a little over a month, or even three modules a week (Monday, Wednesday, and Friday) for a 3-month term. It is likely that as you then move on to actually begin using a cryo-EM or otherwise engage in the field, you will want to repeat certain modules....

최상위 리뷰

대학: LR

Feb 19, 2018

Great course. I thought it was presented in a very informative way and i learned a lot about cryo-EM. In fact, it made me want to learn more about it. Thank you.

대학: AL

Jul 20, 2016

An excellent introduction to the topic, explaining from the basics to the detail of things. Useful and recommended to anyone in structural biology.

필터링 기준:

42개의 리뷰

대학: Naďa Labajová

Mar 22, 2019

Great course. Pity is that you don't give the certificate. Anyway, I'd like to thank to prof. Jensen for it.


Sep 12, 2018

very good for the beginner like me

대학: William Navarre

Aug 31, 2018

Loved it. Although it's not heavy on math it helps to have a basic understanding of waves, how they propagate, how they are mathematically represented by sin/cos functions and how interference between two waves works.

대학: Gerardo Martinez Herrera

Mar 28, 2018

There is a few free courses, this one of the best that I've taken. It would be better if one finishes the course, one could receive any certification

대학: Lance Richardson

Feb 19, 2018

Great course. I thought it was presented in a very informative way and i learned a lot about cryo-EM. In fact, it made me want to learn more about it. Thank you.

대학: Nikos Nikolopoulos

Oct 30, 2017

CryoEM made crystal clear! ;P

대학: stanwang

Oct 19, 2017


대학: Vadim Sukhorukov

Jun 23, 2017

Great job! Clear explanations, drawings, schemes.

대학: Jonathan Foster

May 18, 2017

Great course, very informal - really improved my knowledge in the field of EM

대학: Robert Fagiewicz

Apr 04, 2017

Great course with clear explanations and very entertaining topics! Didn't expect to cover so many aspects of cryo-EM, would recommend it to everyone interested/working in structural biology or science in general.