Chevron Left
Basics of Extracellular Vesicles(으)로 돌아가기

캘리포니아 대학교 어바인 캠퍼스의 Basics of Extracellular Vesicles 학습자 리뷰 및 피드백

4.8
별점
319개의 평가

강좌 소개

This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea)....

최상위 리뷰

MH

2019년 1월 10일

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.

DD

2020년 5월 16일

I found Ia very professional way to introduce everyone to the amazing world of EV. Professor show elegantly their knowledge. The course fulfils my expectations. Congratulations! Nice works

필터링 기준:

Basics of Extracellular Vesicles의 87개 리뷰 중 51~75

교육 기관: Petr N

•

2020년 10월 10일

Very useful course not only for newbies in the EV field. Thank you

교육 기관: Daniel .

•

2022년 4월 3일

It´s a great course to people that starting to study of vesicles.

교육 기관: Asmaa A M A

•

2020년 5월 14일

Excellent course, totally enjoyed it and benefited alot from it

교육 기관: Laura M M E

•

2020년 5월 4일

Excelente curso, expositores con amplio conocimiento del tema

교육 기관: Stephanie B

•

2019년 10월 5일

Good basic overview for someone starting in their studies

교육 기관: Wenhao L

•

2017년 4월 26일

Very wonderful courses! Better than reading 100 papers!

교육 기관: Mohammad R

•

2017년 2월 5일

This course gives an excellent overview of the field!

교육 기관: Nahla I S H

•

2020년 4월 22일

A very good course, really it was very informative.

교육 기관: pbmansfield

•

2017년 3월 6일

Well put together and very informative. Great job!

교육 기관: Dr.� S R S A P -

•

2020년 6월 26일

very helpful and nice videos and the contents too

교육 기관: Ramiro R d l C

•

2020년 11월 4일

Great! Very informative and I learned a lot.

교육 기관: Emilio E S

•

2021년 7월 26일

really good course. I learn a lot about EVs

교육 기관: Regina C

•

2020년 12월 29일

Very interesting and simple to understand

교육 기관: Olivia C

•

2020년 8월 6일

very useful course, I really enjoy it!

교육 기관: Yingchao M

•

2020년 4월 9일

Very good for beginners.

교육 기관: David H

•

2020년 9월 1일

excellent course

교육 기관: Ábner M N

•

2019년 11월 21일

Amazing course!

교육 기관: Dr T S R

•

2020년 9월 17일

Super Course

교육 기관: Adel D

•

2016년 11월 3일

very good!

교육 기관: CAROLINA T P

•

2021년 7월 30일

Excellent

교육 기관: ganesha p

•

2020년 9월 16일

Excellent

교육 기관: Lee-Ann M C

•

2021년 7월 9일

Usefull

교육 기관: Kaio M R

•

2017년 10월 9일

ÓTIMO

교육 기관: Emayamsavitha

•

2020년 4월 27일

Gud

교육 기관: PRIYASHREE N

•

2020년 7월 3일

The way the course was introduced and was taught is really amazing. There were few issues in understanding the audio and there was problem with subtitles as well but still it was a good and amazing experience to meet and listen to researchers from various parts of world. Though it is a blooming field, there was a lot of information in this course. And I gained lot of information from this course. Thanks for every single researcher and professor who are involved in this course. It was a great pleasure to meet and hear your lectures.

Coursera Footer

경력을 시작하거나 쌓기

인기 있는 주제 찾아보기

인기 강좌 및 문서

온라인으로 학위 또는 자격증 취득

Coursera

커뮤니티

기타

어디에서나 학습
앱스토어에서 다운로드하기구글 플레이에서 받기
Placeholder