Chevron Left
Basics of Extracellular Vesicles(으)로 돌아가기

캘리포니아 대학교 어바인 캠퍼스의 Basics of Extracellular Vesicles 학습자 리뷰 및 피드백

4.8
별점
319개의 평가

강좌 소개

This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea)....

최상위 리뷰

MH

2019년 1월 10일

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.

DD

2020년 5월 16일

I found Ia very professional way to introduce everyone to the amazing world of EV. Professor show elegantly their knowledge. The course fulfils my expectations. Congratulations! Nice works

필터링 기준:

Basics of Extracellular Vesicles의 87개 리뷰 중 76~87

교육 기관: Arjun J

2021년 9월 7일

Definitely got to learn quite a bit of possibly useful stuff (I'm very new to this) during the course. The quality of lectures varied on a spectrum from average to great, depending on the instructor for each topic. While some of them literally just read off the slides they prepared, others delivered the info with evident passion, which kept you attentive and interested. It's a mixed bag, but don't let that stop you - overall, the course experience was not bad.

교육 기관: Mainak B

2019년 11월 2일

Excellent content for learning about basic of extracellular vesicles beginners. One thing I would like to point out is it would have been great if the course materials are made available as a pdf version.

교육 기관: Tim D R

2022년 5월 25일

A lot of information broken down in logical and short segments (30 minutes) with great overviews of what each module will cover.

교육 기관: Enrique V O

2017년 5월 11일

It is good much info about vesicles that you can't get from the books and techniques to study them.

교육 기관: Shalmali P

2018년 3월 3일

Very nice nd inormative presentations. But few words in many presentations are not audible.

교육 기관: Marine H

2020년 8월 11일

The last two modules were extremely helpful and a very good opener to this topic.

교육 기관: David d V L

2018년 3월 29일

Excellents classes I have learned a lot of procotols about EVs

교육 기관: paula s m c

2022년 4월 4일

Es un curso muy completo y bien orientado.

교육 기관: Xiaokun L

2017년 7월 20일

nice

교육 기관: Alexander Z

2020년 10월 7일

Overall, pretty good background information. But some of these lectures need to be a bit more audible. I only had one issue with a quiz in which it felt like the question asked either wasn't covered or emphasized as important to the overall subject.

교육 기관: lcy9086

2018년 4월 1일

I am sorry to say that many parts of the lecture provided by the Korean professor is inaudible......SO you can only learn from his slides :(

교육 기관: Yusuf M A

2021년 4월 21일

Cannot understand the instructors.