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지금 바로 시작해 나만의 일정에 따라 학습을 진행하세요.

유동적 마감일

일정에 따라 마감일을 재설정합니다.

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강의 계획 - 이 강좌에서 배울 내용

1
완료하는 데 3시간 필요

From sampling to sequencing

In this module, you will be introduced to metagenomics, some of the considerations and controls that need to be in place in a metagenomics study, and to the topic of antimicrobial resistance. You will also learn about: 1) Sampling and sample handling - the considerations behind a sampling plan, how to perform sampling in practice, and how sample storage can affect metagenomics results; 2) DNA and RNA extraction methods - both for bacterial and viral microorganisms; 3) Sequencing - from library preparation to the basics of different sequencing technologies.

...
10 videos (Total 112 min), 13 readings, 2 quizzes
10개의 동영상
Introduction to antimicrobial resistance11m
Sampling for surveillance18m
Sampling at farms and slaughterhouses14m
Sample storage2m
Isolation of DNA from complex samples10m
Sample processing for viral metagenomics7m
Sequencing platforms12m
13개의 읽기 자료
Glossary20m
Get started !5m
Introduction to metagenomics2m
Considerations and controls for metagenomic/microbiome projects2m
Introduction to antimicrobial resistance2m
Sampling for surveillance5m
Sampling at farms and slaughterhouses2m
Sample storage2m
Isolation of DNA from complex samples2m
Notes on library preparation5m
Sequencing platforms10m
Assessment case-study - part 15m
Assessment case study - part 22m
2개 연습문제
Module 1 assessment - part 18m
Module 1 assessment - part 212m
2
완료하는 데 3시간 필요

FROM READS TO RESULTS

In this module, you will be introduced to the basics of bioinformatics analysis of metagenomics data, including the different types of analysis possible and the different algorithms available. You will then learn about quality control, MGmapper and KRAKEN (two freely available bioinformatics pipelines), and ResFinder (a database of antimicrobial resistance genes). An example from a real study will demonstrate some of the challenges of a bioinformatics analysis.

...
6 videos (Total 64 min), 11 readings, 1 quiz
6개의 동영상
ResFinder database5m
Demo of metagenomic classification using KRAKEN16m
Real example of metagenomic analysis - lessons learned14m
11개의 읽기 자료
General introduction to bioinformatics analysis of metagenomics data5m
Overview of available metagenomic analysis tools10m
MGmapper10m
MGmapper - Best mode vs Full mode10m
ResFinder database3m
Assessment case-study - part 310m
Samples sheet10m
Report A - FastQC results10m
Report B - MGmapper results10m
Report C - KRAKEN results10m
Report D10m
1개 연습문제
Module 2 assessment24m
3
완료하는 데 8시간 필요

Interpretation of results and potential of metagenomics for surveillance

In this module you will learn about two different approaches to analyse and interpret sequence reads - classification and assembly. You will see examples of methods to vizualize read counts and to analyze metagenomics together with explanatory data. Last, you will learn the potential of metagenomics for the development of a future global and integrated surveillance, and the challenges you may encounter during that process

...
6 videos (Total 81 min), 16 readings, 6 quizzes
6개의 동영상
Application of metagenomics in surveillance - methods (part 2)16m
Application of metagenomics in surveillance - opportunities and challenges21m
Farewell3m
16개의 읽기 자료
Virtual Machine setup1h
Analysis and visualization of read count data10m
Metagenomic assembly and binning: reconstructing genomes from reads10m
Application of metagenomics in surveillance - methods10m
Potential of metagenomics for surveillance10m
Challenges of metagenomics for surveillance10m
Assessment case-study - part 45m
Report D10m
Epidemiology sheet and Report E10m
Introduction5m
Final quiz - section I - Sampling10m
Final quiz - section II - Quality control30m
Final quiz - section III - Bioinformatics analysis of metagenomics30m
Final quiz - section IV - Interpretation of metagenomics results10m
Final quiz - section V - Final quiz - section V - Analysis of metagenomics results in a surveillance context10m
Extra material for final assessment10m
6개 연습문제
Module 3 assessment10m
Final quiz - section I - Sampling18m
Final quiz - section II - Quality control30m
Final quiz - section III - Bioinformatics analysis of metagenomics30m
Final quiz - section IV - Interpretation of metagenomics results30m
Final quiz - section V - Analysis of metagenomics results in a surveillance context30m

강사

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Ana Sofia Ribeiro Duarte

Assistant Professor
National Food Institute
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Sünje Johanna Pamp

Associate Professor
Research Group for Genomic Epidemiology, Technical University of Denmark
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Liese Van Gompel

PhD Candidate Epidemiology
Utrecht University, Institute for Risk Assessment Sciences (IRAS)
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Pimlapas Leekitcharoenphon

PostDoc
Research Group for Genomic Epidemiology, DTU Food

덴마크 기술 대학교 (DTU) 정보

DTU focuses on research in technical and natural sciences that contributes to the development of society. As an industrially orientated university, our goal is to supply high-level international research based on combining theory with constructing models and empirical methods....

자주 묻는 질문

  • 강좌에 등록하면 바로 모든 비디오, 테스트 및 프로그래밍 과제(해당하는 경우)에 접근할 수 있습니다. 상호 첨삭 과제는 이 세션이 시작된 경우에만 제출하고 검토할 수 있습니다. 강좌를 구매하지 않고 살펴보기만 하면 특정 과제에 접근하지 못할 수 있습니다.

  • 수료증을 구매하면 성적 평가 과제를 포함한 모든 강좌 자료에 접근할 수 있습니다. 강좌를 완료하면 전자 수료증이 성취도 페이지에 추가되며, 해당 페이지에서 수료증을 인쇄하거나 LinkedIn 프로필에 수료증을 추가할 수 있습니다. 강좌 콘텐츠만 읽고 살펴보려면 해당 강좌를 무료로 청강할 수 있습니다.

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