About this Course
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지금 바로 시작해 나만의 일정에 따라 학습을 진행하세요.

탄력적인 마감일

일정에 따라 마감일을 재설정합니다.

초급 단계

완료하는 데 약 13시간 필요

권장: 12-18 hours videos and labs...

영어

자막: 영어

귀하가 습득할 기술

Genetic AnalysisBioinformatics AnalysisEvolutionComparative Genomics

100% 온라인

지금 바로 시작해 나만의 일정에 따라 학습을 진행하세요.

탄력적인 마감일

일정에 따라 마감일을 재설정합니다.

초급 단계

완료하는 데 약 13시간 필요

권장: 12-18 hours videos and labs...

영어

자막: 영어

강의 계획 - 이 강좌에서 배울 내용

1
완료하는 데 3시간 필요

NCBI/Blast I

In this module we'll be exploring the amazing resources available at NCBI, the National Centre for Biotechnology Information, run by the National Library of Medicine in the USA. We'll also be doing a Blast search to find similar sequences in the enormous NR sequence database. We can use similar sequences to infer homology, which is the primary predictor of gene or protein function. ...
4 videos (Total 52 min), 4 readings, 1 quiz
4개의 동영상
Lecture25m
Lab Discussion24m
Summary
4개의 읽기 자료
Acknowledgements10m
Course Logistics10m
Lecture Materials10m
Lab 1 -- Exploring NCBI30m
1개 연습문제
Lab 1 Quiz6m
2
완료하는 데 3시간 필요

Blast II/Comparative Genomics

In this module we'll continue exploring the incredible resources available at NCBI, the National Centre for Biotechnology Information. We will be performing several different kinds of Blast searches: BlastP, PSI-Blast, and Translated Blast. We can use similar sequences identified by such methods to infer homology, which is the primary predictor of gene or protein function. We'll also be comparing parts of the genomes of a couple of different species, to see how similar they are....
4 videos (Total 58 min), 2 readings, 1 quiz
4개의 동영상
Lecture32m
Lab Discussion23m
Summary
2개의 읽기 자료
Lecture Materials10m
Lab 2 -- Advanced Blast and Comparative Genomics30m
1개 연습문제
Lab 2 Quiz6m
3
완료하는 데 2시간 필요

Multiple Sequence Alignments

In this module we'll be doing multiple sequence alignments with Clustal (as implemented in MEGA), DiAlign, and MAFFT. Multiple sequences alignments can tell you where in a sequence the conserved and variable regions are, which is important for understanding the biology of the sequences under investigation. It also has practical applications, such as being able to design PCR primers that will amplify sequences from a number of different species, for example....
4 videos (Total 42 min), 2 readings, 1 quiz
4개의 동영상
Lecture23m
Lab Discussion16m
Summary
2개의 읽기 자료
Lecture Materials10m
Lab 3 -- Multiple Sequence Alignment30m
1개 연습문제
Lab 3 Quiz6m
4
완료하는 데 10분 필요

Review: NCBI/Blast I, Blast II/Comparative Genetics, and Multiple Sequence Alignments

...
1 quiz
1개 연습문제
Quiz: Modules 1-310m
5
완료하는 데 2시간 필요

Phylogenetics

In this module we'll be using the multiple sequence alignments we generated last lab to do some phylogenetic analyses with both neighbour-joining and maximum likelihood methods. The tree-like structure generated by such analyses tells us how closely sequences are related one to another, and suggests when in evolutionary time a speciation or gene duplication event occurred....
4 videos (Total 36 min), 2 readings, 1 quiz
4개의 동영상
Lecture25m
Lab Discussion9m
Summary
2개의 읽기 자료
Lecture Materials10m
Lab 4 -- Phylogenetics30m
1개 연습문제
Lab 4 Quiz6m
6
완료하는 데 2시간 필요

Selection Analysis

In this module we'll take a set of orthologous sequences from bacteria and use DataMonkey to analyze them for the presence of certain sites under positive, negative or neutral selection. Such an analysis can help understand the biology of a set of protein coding sequences by identifying residues that might be important for biological function (those residues under negative selection) or those that might be involved in response to external influences, such as drugs, pathogens or other factors (residues under positive selection)....
4 videos (Total 38 min), 2 readings, 1 quiz
4개의 동영상
Lecture23m
Lab Discussion10m
Summary1m
2개의 읽기 자료
Lecture Materials10m
Lab 5 -- Selection Analysis30m
1개 연습문제
Lab 5 Quiz6m
7
완료하는 데 3시간 필요

'Next Gen' Sequence Analysis (RNA-Seq) / Metagenomics

In this module we'll explore some of the data that have been generated as a result of the rapid decrease in the cost of sequencing DNA. We'll be exploring a couple of RNA-Seq data sets that can tell us where any given gene is expressed, and also how that gene might be alternatively spliced. We'll also be looking at a couple of metagenome data sets that can tell us about the kinds of species (especially microbial species that might otherwise be hard to culture) that are in a given environmental niche....
4 videos (Total 51 min), 2 readings, 1 quiz
4개의 동영상
Lecture24m
Lab Discussion20m
Summary4m
2개의 읽기 자료
Lecture Materials10m
Lab 6 -- Next Generation Sequencing Applications: RNA-Seq and Metagenomics30m
1개 연습문제
Lab 6 Quiz6m
8
완료하는 데 1시간 필요

Review: Phylogenetics, Selection Analysis, and 'Next Gen' Sequence Analysis (RNA-seq)/Metagenomics + Final Assignment

...
1 reading, 2 quizzes
1개의 읽기 자료
Final Assignment Instructions10m
2개 연습문제
Review: Modules 5-710m
Final Assignment10m
4.7
158개의 리뷰Chevron Right

21%

이 강좌를 수료한 후 새로운 경력 시작하기

29%

이 강좌를 통해 확실한 경력상 이점 얻기

20%

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최상위 리뷰

대학: EDApr 27th 2017

Great course. All lectures provide a biological context for the tools you learn in the labs. The labs themselves provide a great introduction to the many tools available for bioinformatic analysis.

대학: FJFeb 6th 2017

The videos were very clear on how to use various bioinformatic tools. I found no difficulty in understanding them. Really enjoyed the course. Looking forward to the next part of this course!

강사

Avatar

Nicholas James Provart

Professor
Cell & Systems Biology

토론토 대학교 정보

Established in 1827, the University of Toronto is one of the world’s leading universities, renowned for its excellence in teaching, research, innovation and entrepreneurship, as well as its impact on economic prosperity and social well-being around the globe. ...

자주 묻는 질문

  • 강좌에 등록하면 바로 모든 비디오, 테스트 및 프로그래밍 과제(해당하는 경우)에 접근할 수 있습니다. 상호 첨삭 과제는 이 세션이 시작된 경우에만 제출하고 검토할 수 있습니다. 강좌를 구매하지 않고 살펴보기만 하면 특정 과제에 접근하지 못할 수 있습니다.

  • 수료증을 구매하면 성적 평가 과제를 포함한 모든 강좌 자료에 접근할 수 있습니다. 강좌를 완료하면 전자 수료증이 성취도 페이지에 추가되며, 해당 페이지에서 수료증을 인쇄하거나 LinkedIn 프로필에 수료증을 추가할 수 있습니다. 강좌 콘텐츠만 읽고 살펴보려면 해당 강좌를 무료로 청강할 수 있습니다.

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