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중급 단계

Basic training in chemistry biology and physics.

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배울 내용

  • Foundational comprehension of chemical biology

  • Interfacial science problem solving and method design

  • Understanding of applied, translational concepts in life sciences

공유 가능한 수료증
완료 시 수료증 획득
100% 온라인
지금 바로 시작해 나만의 일정에 따라 학습을 진행하세요.
유동적 마감일
일정에 따라 마감일을 재설정합니다.
중급 단계

Basic training in chemistry biology and physics.

완료하는 데 약 21시간 필요
영어

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강의 계획 - 이 강좌에서 배울 내용

1

1

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Welcome to Chemical Biology: Concepts and techniques

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8개 동영상 (총 58분), 7 개의 읽기 자료, 4 개의 테스트
8개의 동영상
Welcome1m
Why study chemical biology?11m
Course outline and highlights9m
The building blocks: monomeric units of proteins9m
The building blocks: monomeric units of DNA and membranes8m
Messengers and messages: posttranslational modifications, and pathways8m
Studying cellular processes with chemical biology in real time8m
7개의 읽기 자료
Course Acknowledgements5m
How assessments work10m
The foundation: the cell10m
The workhorses: proteins, enzymes12m
Classical protein readouts12m
Flash mob: overview of protein analysis methods10m
Flash mob - Chemistry15m
4개 연습문제
Components of a cell22m
Amino acids24m
Match technique to goal15m
Final Quiz Number 128m
2

2

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The Devil is in the Minutest Detail

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5개 동영상 (총 37분), 5 개의 읽기 자료, 3 개의 테스트
5개의 동영상
Light'em up! Fluorophores: structures, and fluorescent principles9m
Get out (almost) what you put in!3m
Traditional light microscopy – applications10m
Traditional light microscopy – precautions10m
5개의 읽기 자료
Physics of fluorescence15m
Fluorescent proteins10m
RNA interference and genome editing20m
Plasmids and transfection15m
DNA double stranded breaks and γ-H2AX10m
3개 연습문제
Jablonski diagram16m
Match color of fluorophore to structure20m
Final Quiz Number 222m
3

3

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A ruler over time and space! Fluorescent assays to measure complex parameters in real time

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12개 동영상 (총 88분), 5 개의 읽기 자료, 6 개의 테스트
12개의 동영상
Creating modified chromatin 9m
PRC2, a DNA binding complex linked to development and cancer7m
TIRF talk7m
On our TIRF4m
If your protein surfs on DNA, hit the TIRF to study it8m
PHF1 Prolongs the stable PRC2-DNA complex, promoting activity8m
Fluid mosaic model5m
Don’t get uptight! New methods to measure membrane tension7m
Getting a grip on membrane tension measurements4m
TORCing TORC: the many faces of the TOR proteins8m
TORCing it out: pathways that seek to sense and restore membrane tension13m
5개의 읽기 자료
DNA organization in eukaryotes is linked to regulation10m
Kinetics of chemical reactions6m
Comparing chemical structures6m
Flash mob inteins6m
Model Organisms20m
6개 연습문제
Making proteins via expressed protein ligation and alkylation18m
Hit the tirf: applications of TIRF microscopy11m
Analyzing data from TIRF experiments to gain mechanistic insights17m
Modern fluorescence methods, the spark of creativity15m
TORCing about Tor's role in membrane tension regulation16m
Final Quiz 325m
4

4

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Putting proteins to work for us

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11개 동영상 (총 95분), 2 개의 읽기 자료, 7 개의 테스트
11개의 동영상
Tracking a specific protein in live cells & in real time8m
Molecular zipcodes4m
How to design your fusion protein6m
Design principle and optimization of SNIFITs7m
SNIFITs to ID neurotransmitters and drugs10m
Application to point of care diagnosis7m
Biological electrophiles, the cell’s way of saying “I’m stressed”?8m
Breaking the tether is not that bad10m
T-REX: a question of control(s)16m
T-REX rewiring of kinase activity through targeting AKT and PTEN: opposing forces meet 10m
2개의 읽기 자료
Many enzyme mechanisms proceed through a covalent enzyme intermediate that can be stabilized through mutagenesis10m
Small molecule signals8m
4개 연습문제
Optimal considerations for making fusion proteins and employing them in chemical biology24m
Optimal considerations for making fusion proteins and employing them in chemical biology214m
A bit on the nose? Chose the right SNIFITs20m
Final Quiz Number 424m

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