About this Course
4.7
136개의 평가
32개의 리뷰

100% 온라인

지금 바로 시작해 나만의 일정에 따라 학습을 진행하세요.

탄력적인 마감일

일정에 따라 마감일을 재설정합니다.

중급 단계

완료하는 데 약 13시간 필요

권장: 5 weeks, 2-3 hours/week...

영어

자막: 영어

귀하가 습득할 기술

BiologySample PreparationSample CollectionCell Biology

100% 온라인

지금 바로 시작해 나만의 일정에 따라 학습을 진행하세요.

탄력적인 마감일

일정에 따라 마감일을 재설정합니다.

중급 단계

완료하는 데 약 13시간 필요

권장: 5 weeks, 2-3 hours/week...

영어

자막: 영어

강의 계획 - 이 강좌에서 배울 내용

1
완료하는 데 2시간 필요

Introduction to the Course and the Field of Extracellular Vesicles

In this module, we will introduce the course and the field of extracellular vesicles. We will discuss the nomenclature and the history of extracellular vesicles as well as the organisms and places that have been shown to release/contain extracellular vesicles. ...
4 videos (Total 69 min), 1 reading, 1 quiz
4개의 동영상
Introduction to the Course11m
The Origin of EVs throughout the Phylogenetic Tree11m
History of Exosomes and Extracellular Vesicles32m
1개의 읽기 자료
Syllabus10m
1개 연습문제
Introduction to Extracellular Vesicles18m
2
완료하는 데 3시간 필요

Biogenesis, Cargo and Uptake of Extracellular Vesicles

In this module, we will focus on the biogenesis and release of extracellular vesicles as well as the uptake mechanisms of extracellular vesicles when they are encountered by a recipient cell. This module also covers the different RNA, protein and lipid types present in extracellular vesicles as well as a brief overview what the functions of these molecules are. Furthermore, the techniques that are commonly used to detect these molecules as well as databases that can be useful to use when analyzing the cargo of extracellular vesicles will be highlighted....
6 videos (Total 142 min), 1 quiz
6개의 동영상
Mechanisms of Extracellular Vesicle Uptake - Part 122m
Mechanisms of Extracellular Vesicle Uptake - Part 223m
The Protein Content of Extracellular Vesicles23m
The RNA Content of Extracellular Vesicles35m
The Lipid Content of Extracellular Vesicles18m
1개 연습문제
Biogenesis, Cargo and Uptake of Extracellular Vesicles30m
3
완료하는 데 2시간 필요

Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles

In this module, we will focus on the collection and processing of cell culture media and body fluids prior to isolation of extracellular vesicles. We will discuss some of the considerations and guidelines that are important during the collection of staring material and isolation of extracellular vesicles. This module will help you with the different choices, such as anticoagulants, collection time points and protein inhibitors, which are important for the outcome when working with a particular body fluid compared to conditional media or other body fluids. This module will also illustrate some example of studies on extracellular vesicles from body fluid such as blood, urine, breast milk and cerebrospinal fluid and why it is of interest to analyse extracellular vesicles from these body fluids. ...
5 videos (Total 79 min), 1 quiz
5개의 동영상
Blood Plasma and Serum15m
Breast Milk8m
Urine25m
Cerebrospinal Fluid15m
1개 연습문제
Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles36m
4
완료하는 데 2시간 필요

Methods for Isolating Extracellular Vesicles

This module highlights some of the different methods used to isolate extracellular vesicles. Here the basic concepts for methods such as differential ultracentrifugation, density gradient, size exclusion and kit-based precipitation will be covered and some guidelines will be presented. Furthermore, this module will cover how the techniques are used in the field of extracellular vesicles as well as their limitations and benefits....
6 videos (Total 77 min), 1 quiz
6개의 동영상
Differential Ultracentrifugation - Part 216m
Density Gradient12m
Size Exclusion Chromatography14m
Kit-based Precipitation12m
Summary of Isolation Methods for Extracellular Vesicles6m
1개 연습문제
Methods for Isolating Extracellular Vesicles22m
4.7
32개의 리뷰Chevron Right

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이 강좌를 수료한 후 새로운 경력 시작하기

최상위 리뷰

대학: MHJan 11th 2019

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.

대학: MVJan 31st 2018

The only problem I faced, has to do with the subtitles. They have lots of mismatches and mistakes. Nevertheless, the MOOC is undoubtedly great and pretty educational.

강사

Avatar

Cecilia Lasser

Researcher
Krefting Research Centre, University of Gothenburg

캘리포니아 대학교 어바인 캠퍼스 정보

Since 1965, the University of California, Irvine has combined the strengths of a major research university with the bounty of an incomparable Southern California location. UCI’s unyielding commitment to rigorous academics, cutting-edge research, and leadership and character development makes the campus a driving force for innovation and discovery that serves our local, national and global communities in many ways....

자주 묻는 질문

  • 강좌에 등록하면 바로 모든 비디오, 테스트 및 프로그래밍 과제(해당하는 경우)에 접근할 수 있습니다. 상호 첨삭 과제는 이 세션이 시작된 경우에만 제출하고 검토할 수 있습니다. 강좌를 구매하지 않고 살펴보기만 하면 특정 과제에 접근하지 못할 수 있습니다.

  • 수료증을 구매하면 성적 평가 과제를 포함한 모든 강좌 자료에 접근할 수 있습니다. 강좌를 완료하면 전자 수료증이 성취도 페이지에 추가되며, 해당 페이지에서 수료증을 인쇄하거나 LinkedIn 프로필에 수료증을 추가할 수 있습니다. 강좌 콘텐츠만 읽고 살펴보려면 해당 강좌를 무료로 청강할 수 있습니다.

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